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Development of new universal industrial biotechnology of ecologically hazardous food wastes recycling and neutralization. PDF Print E-mail

We developed the scientific basis of solid food waste utilization using biotechnologies that utilize the metabolism regulation of microorganisms-destructors. We identified that mass transfer intensity in cultivator determines technological characteristics of food wastes destruction. The comparative analysis of these characteristics in our modified cultivators (mechanic shaking, impulse hydraulic shaking and without shaking) showed that the most efficient is food wastes destruction with hydraulic shaking: destruction coefficient increased four times, time of destruction decreased almost two times.  Complex tests of pilot plant were conducted by us. We identified that almost complete destruction of food wastes is achieved in 9 days, when using impulse hydraulic shaken cultivator along with microorganisms-destructors metabolism regulating biotechnology. Besides, biogas, including hydrogen (61%) and methane (35%) had been emitting in this process. This makes this biotechnology useful both for solid food waste neutralization and biogas extraction.

Developing the new biotechnology of residential waste water treatment under low-temperature conditions. PDF Print E-mail

For the first time we developed the biotechnology of hyperhaline (with 35 g/l sea salt concentration) waste water treatment from anthropogenic pollution for ecologically clean environmental regions (Arctic, Antarctica). The biotechnology presumes using the indigenous microbial associations, isolated from sea silt. We created the pilot plant and defined the stereometrical parameters of the module and the carriers of microorganisms-organic compound destructors as well as control technological parameters of the treating process. Using of this biotechnology in a flowing module allowed decreasing organic compound concentration in 15 times. The biotechnology developed by us is efficient and ecologically safe and may be used for domestic wastewater treatment on Ukrainian Antarctic station Academician Vernadsky.

Autecology, taxonomy and biotechnical potential of microorganisms assimilating hydrocarbons and their derivatives. PDF Print E-mail

We defined the new species of Methylomonas rubra based on polyphase analysis. The denomination of the species Methylomonas rubra sp. nov. is legalized according to the rules of International Codex of bacterial nomenclature. Methylotrophic strains "Methylobacter ucrainicus" are reclassified by us as Methylobacter marinus, several strains are identified as Methylococcus capsulatus.
For the first time we identified wide spreading of heterotrophic metal-resistant microorganisms in Antarctica. In most terrestrial biotopes of Antarctic regions examined e found microorganisms resistant to heavy metals (100-500 mg ions Сr, Cu, Cd or Hg). Practically at all the islands of Argentina Archipelago we found the Methylobacterium strains. They were found in different biotopes, but most frequently in moss and lichens.
We defined the possibility of oil-contaminated (100-200 mg/l) water treatment at the pilot plant with Rhodococcus erythropolis and Nocardia vaceinii cells immobilized on haydite. We defined the optimal conditions of cultivating Rhodococcus erythropolis on hexadecane for surface active agents (SAA) production were. The synthesis of SAA under these conditions increases 3-4 times.

Structure functional characteristics of microbial cenosises in Antarctica. Studying the role of microorganisms in biogeochemical cycles. PDF Print E-mail

We analyzed samples from different biotopes and regions of western Antarctica. We found that methylotrophic microorganisms are widely spread in lichens, grass and mosses as well as in soils of Antarctica. Their quantity in different Antarctic biotopes is comparable with one in temperate climate regions (Ukraine for example). We did not find metal-resistant methylotrophic microorganisms in Antarctica.  Yet, in most of terrestrial biotopes (soils, mosses, lichens, lake silt, water, humus) of examined Antarctic regions we have found microorganisms resistant to high concentrations of heavy metals (100…500 mg ions per liter Сr, Cu, Cd or Hg). Thus, for the first time we identified the wide spreading of high concentration of heavy metal resistant microorganisms in Antarctica. The phenomenon originality lies in combination of:

•microorganisms’ ability to grow on high concentrations of heavy metals;
•incomparably low concentrations of heavy metals in Antarctic samples (from 0,02 to 40 mg/kg);
•metal resistant microorganisms presence in all terrestrial biotopes of Antarctica;
•high quantity of metal resistant microorganisms in these biotopes (104 … 105 cells/g of sample).

Antialcohol and antidrug biological preparation. PDF Print E-mail

On the living biomass of alcohol-consuming microorganisms we developed a biological preparation for treating and preventing alcohol and drug abuse. Our experiments show that the preparation affects organism regulatory systems, turning into normal level specific physiological and biochemical characteristics, affected by alcohol and drugs, namely alcohol and aldehyde dehydrogenase, adrenalin, noradrenaline and dopamine. The biopreparation decreases alcohol and opiate intoxication, prevents alcohol and drug abuse progress, lessens complications, determined by chronic alcoholism (cirrhosis, spasms). The preparation is not the product of chemical synthesis, but a complex of natural agents, including proteins, amino acids, vitamins, carotinoides, microelements and other biologically active agents necessary for full organism vital functions. The preparation has not shown any pathogenic or toxic affects. We developed the biotechnology for production of the preparation. No scarce materials are used for production of our preparation. The preparation development is patented in Ukraine. We need investments for industrial production organization and for implementation in medical practice.

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